waarnemingsrecord: 2021_November_130_RUN2:MiFish_UE-asv10280

Material Sample van Bacteria opgenomen op 2021-11-23
   API

Dataset

Gegevenspartner Flanders Research Institute for Agriculture, Fisheries and Food (ILVO)
Gegevensbron 12S eDNA from seawater samples collected within the coastal zone of the Belgian part of the North Sea
WaarnemingsID (dwc:occurrenceID) 2021_November_130_RUN2:MiFish_UE-asv10280
Record type Material Sample
License CC-BY 4.0 (Int)
Geleverd als CC_BY_4_0
Presence/Absence PRESENT
Organisme kwantiteit (dwc:organismQuantity) 9
Materiaal monster ID (dwc:materialSampleID) https://www.ncbi.nlm.nih.gov/biosample/SAMN42316027 | https://www.ncbi.nlm.nih.gov/biosample/SAMN42316126 | https://www.ncbi.nlm.nih.gov/biosample/SAMN42316225
Organisme kwantiteit type (dwc:organismQuantityType) DNA sequence reads
Bemonsteringsprotocol (dwc:samplingProtocol) [Niskin bottles]
Identificatie referenties (dwc:identificationReferences) the custom made 12S reference db can be found atDOI 10.5281/zenodo.13861942
Identificatie opmerkingen (dwc:identificationRemarks) Taxonomy was assigned in three steps on the merged dataset. First, taxonomy was assigned using a custom-made database (12S reference sequences of 122 North Sea fish species; available at  DOI 10.5281/zenodo.13861942) and the naïve Bayesian RDP Classifier (minBoot = 80; Wang et al., 20017). Secondly, unassigned ASVs after the first step were run with blastn v2.12.0 (Altschul et al., 1990) against the custom-made database. In the third step, unassigned ASVs were subjected to BLAST and the GenBank nt database (from August 2023; qcov_hsps_perc= 75%; perc_identity = 97%). The taxonomy for species with identical 12S sequences was set to genus level (Alosa alosa and Alosa fallax, Raja brachyura, Raja montagui, and Raja clavata) or family level (Ammodydes tobianus and Ammodytes marinus, Chelidonichthys lucerna, Chelidonichthys cuculus, and Eutrigla gurnardus). Other ASVs for which taxonomy could not be determined to species level were classified to family or genus level.

Gebeurtenis

Event ID 2021_November_130_RUN2
Identificatie opmerkingen (dwc:identificationRemarks) Taxonomy was assigned in three steps on the merged dataset. First, taxonomy was assigned using a custom-made database (12S reference sequences of 122 North Sea fish species; available at  DOI 10.5281/zenodo.13861942) and the naïve Bayesian RDP Classifier (minBoot = 80; Wang et al., 20017). Secondly, unassigned ASVs after the first step were run with blastn v2.12.0 (Altschul et al., 1990) against the custom-made database. In the third step, unassigned ASVs were subjected to BLAST and the GenBank nt database (from August 2023; qcov_hsps_perc= 75%; perc_identity = 97%). The taxonomy for species with identical 12S sequences was set to genus level (Alosa alosa and Alosa fallax, Raja brachyura, Raja montagui, and Raja clavata) or family level (Ammodydes tobianus and Ammodytes marinus, Chelidonichthys lucerna, Chelidonichthys cuculus, and Eutrigla gurnardus). Other ASVs for which taxonomy could not be determined to species level were classified to family or genus level.
Datum van waarneming 2021-11-23
Geleverd als jaar:2021  maand:11  dag:23 
Bemonsteringsprotocol (dwc:samplingProtocol) Niskin bottles  
Einddag van het jaar 327
Precisie datum DAY
Startdag van het jaar (dwc:startDayOfYear) 327

Taxonomie

Scientific name Bacteria
Supplied scientific name "uncultured bacterium"
Identified to rank kingdom
Rijk Bacteria
Stam NA
Class NA
Orde NA
Familie NA
Genus NA
Name match metric higherMatch
Name parse type PLACEHOLDER
Taxonomische status (dwc:taxonomicStatus) ACCEPTED

Geospatiaal

Land Belgium
Latitude 51.2708
Supplied as: "51.2708"
Longitude 2.905
Supplied as: "2.905"
Datum EPSG:4326
Supplied datum: "WGS84"
Coordinate precision Unknown
Terrestrial true
Biome TERRESTRIAL
Continent EUROPE
Marine false
Country Code BE

Aanvullende eigenschappen

accepted scientific name Bacteria
accepted taxon key 3
core id 4997051939
dataset key 8ddac24b-e022-46df-8883-11e4f222142b
gbif id 4997051939
has geospatial issues false
kingdom key 3
last crawled 2025-08-07T22:37:29.060Z
last interpreted 2025-08-07T22:38:18.605Z
last parsed 2025-08-07T22:38:18.605Z
protocol DWC_ARCHIVE
taxon key 3
verbatim scientific name uncultured bacterium

Door gebruiker gemarkeerde problemen 

    Gegevenskwaliteitscontroles

    Testnaam Resultaat
    CONTINENT_COORDINATE_MISMATCH Waarschuwing
    Coordinate uncertainty meters invalid Waarschuwing
    Invalid scientific name Waarschuwing
    Taxon match higher rank Waarschuwing
    Weergeven/Verbergen 81 passed properties
    Weergeven/Verbergen 5 missing properties
    Weergeven/Verbergen 34 tests that have not been run

    Aanvullende informatie over politieke grenzen

    Administratief
    Vlaanderen inclusief de exclusief economische zone Vlaams Gewest
    Hydrografie
    Belgische Exclusieve economische zone {D9B06511-41DD-4968-91B8-D11ED20F5476}
    Locatie
    Meetpunten VMM buffer 0,001 ° NZ130
    UTM 5 DS97A